1. Which of the following is accurate with respect to

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1. Which of the following is accurate with respect to RNA synthesis in prokaryotes? A) Ribonucleotides are the monomers that are initially required, and they pair with complementary bases on the template strand of DNA. B) Basal transcription factors initiate transcription by binding to the appropriate promoter region in DNA. C) The regulatory protein sigma orients the DNA template inside RNA Polymerase. D) The unique sequence approximately 30 base pairs upstream of the transcription start site called the TATA box helps orient where transcription should begin. E) All of the above (select ‘E’ if A-D are all correct). 2. During initiation of translation, the first codon, _________, enters the _______site, and associates with the _________. A) UAG, A site, ribosome binding site B) AUG, ribosome binding site, initiator aminoacyl tRNA C) UAG, P site, initiator aminoacyl tRNA D) AUG, P site, initiator aminoacyl tRNA E) AUG, P site, ribosome binding site. 3. What are small nuclear ribonucleoproteins, where would they be found, and what do they do? A) protein plus RNA complexes, found in the spliceosome in the cell’s nucleus, edit mRNA by removing introns B) small nuclear RNAs, found in the cells’ nucleus, are involved in adding caps and tails to form mature mRNA C) active RNA transcripts complexed with protein, found in spliceosome in cell’s nucleus, are involved in adding caps and tails to form mature mRNA D) small nuclear RNAs, found in the splicesome in the cell’s cytoplasm, edit mRNA by removing introns E) protein plus RNA complexes, found in the ribosomes, edit mRNA by removing introns 4. Which of the following is TRUE about tRNA? A) The “wobble hypothesis” explains why just 40 tRNAs are capable of translating 61 mRNA codons. B) The nucleotide sequence ‘CCA’ serves as binding site for amino acids and juts out at the 5’ end of the tRNA. C) The ‘loops’ in the tRNA are double-stranded. D) Aminoacyl tRNA is the name for the ‘uncharged’ form of tRNA. E) All of the above (select answer ‘E’ if A-D are all true). 5. Given the DNA template shown in the figure below, which of the following bases would you find in a complementary RNA strand and where in the eukaryotic cell would they be synthesized? Given the DNA template shown in the figure above, which of the following bases would you find in a complementary RNA strand and where in the eukaryotic cell would they be synthesized? A. U-U-U-U-U; ribosome B. U-U-U-U-U; nucleus C. A-A-A-A-A; ribosome D. A-A-A-A-A; nucleus E. None of the above; a primer is needed to initiate this synthesis and one isn’t provided

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